c perfringens reference strain atcc 13124  (ATCC)

Journal: Pathogens (Basel, Switzerland)

Article Title: Addressing Current Challenges in Poultry Meat Safety: Development of a Cultivation and Colony Hybridization Approach to Recover Enterotoxigenic Clostridium perfringens from Broiler Chicken Carcasses.

doi: 10.3390/pathogens13010030

Figure Lengend Snippet: Figure 3. Nylon membrane after hybridization between the labeled probe and the DNA from C. perfringens control strains used in this study (AHL 311 and ATCC 13124); positive hybridization signals were observed for AHL 311 only. Red square is showing the DNA demonstrating a positive hybridization signal.

Article Snippet: Three distinct DNA extractions were performed: two DNA extractions from the cpepositive C. perfringens AHL 311 control strain that yielded 27.9 ng/μL and 33.3 ng/μL of DNA and one DNA extraction from the cpe-negative ATCC 13124 C. perfringens reference strain yielding 28.0 ng/μL of DNA.

Techniques: Membrane, Hybridization, Labeling, Control

Journal: Pathogens (Basel, Switzerland)

Article Title: Addressing Current Challenges in Poultry Meat Safety: Development of a Cultivation and Colony Hybridization Approach to Recover Enterotoxigenic Clostridium perfringens from Broiler Chicken Carcasses.

doi: 10.3390/pathogens13010030

Figure Lengend Snippet: Figure 4. Hybridization from pure lysed colonies of C. perfringens reference strains positive and negative for the cpe gene (AHL 311 and ATCC 13124). (A) Bacterial growth on a blood agar. (B) Nylon membrane after hybridization between the labeled probe and lysed colonies of C. perfringens reference strains used in this study; positive hybridization signals were observed from AHL 311 lysed colonies, whereas no hybridization signal was generated for ATCC 13124. Red squares are showing colonies demonstrating a positive hybridization signal.

Article Snippet: Three distinct DNA extractions were performed: two DNA extractions from the cpepositive C. perfringens AHL 311 control strain that yielded 27.9 ng/μL and 33.3 ng/μL of DNA and one DNA extraction from the cpe-negative ATCC 13124 C. perfringens reference strain yielding 28.0 ng/μL of DNA.

Techniques: Hybridization, Membrane, Labeling, Generated

Journal: Pathogens (Basel, Switzerland)

Article Title: Addressing Current Challenges in Poultry Meat Safety: Development of a Cultivation and Colony Hybridization Approach to Recover Enterotoxigenic Clostridium perfringens from Broiler Chicken Carcasses.

doi: 10.3390/pathogens13010030

Figure Lengend Snippet: Figure 5. Hybridization from lysed colonies grown from a sterile freezing medium artificially contaminated with C. perfringens AHL 311. (A) Bacterial growth of C. perfringens AHL 311 from sample tube 4 on one of the 10 hydrophobic grids laid on TSC agar plates. (B) Positive hybridization signals from cpe-positive colonies of C. perfringens AHL 311 added to tube 4 containing a sterile freezing medium filtered on hydrophobic grids incubated on TSC agar plates and printed on nylon membranes. The positive control corresponds to the cpe PCR product. Red square is showing colonies demonstrating a positive hybridization signal.

Article Snippet: Three distinct DNA extractions were performed: two DNA extractions from the cpepositive C. perfringens AHL 311 control strain that yielded 27.9 ng/μL and 33.3 ng/μL of DNA and one DNA extraction from the cpe-negative ATCC 13124 C. perfringens reference strain yielding 28.0 ng/μL of DNA.

Techniques: Hybridization, Sterility, Incubation, Positive Control

Journal: Pathogens (Basel, Switzerland)

Article Title: Addressing Current Challenges in Poultry Meat Safety: Development of a Cultivation and Colony Hybridization Approach to Recover Enterotoxigenic Clostridium perfringens from Broiler Chicken Carcasses.

doi: 10.3390/pathogens13010030

Figure Lengend Snippet: Figure 7. Application of the HGMF-CH method using samples of broiler chicken carcass rinsates artificially contaminated with C. perfringens AHL 311 (tube 3, Table 2). (A) C. perfringens growth on a hydrophobic grid incubated on TSC agar plates after the filtration of a broiler chicken carcass rinsate artificially contaminated with C. perfringens AHL 311. (B) Positive hybridization signals on a nylon membrane printed from TSC agar plates with C. perfringens growth (A) were filtered. The positive control (cpe PCR product) appears on the nylon membrane. Red squares are showing colonies demonstrating a positive hybridization signal.

Article Snippet: Three distinct DNA extractions were performed: two DNA extractions from the cpepositive C. perfringens AHL 311 control strain that yielded 27.9 ng/μL and 33.3 ng/μL of DNA and one DNA extraction from the cpe-negative ATCC 13124 C. perfringens reference strain yielding 28.0 ng/μL of DNA.

Techniques: Incubation, Filtration, Hybridization, Membrane, Positive Control

Journal: Pathogens (Basel, Switzerland)

Article Title: Addressing Current Challenges in Poultry Meat Safety: Development of a Cultivation and Colony Hybridization Approach to Recover Enterotoxigenic Clostridium perfringens from Broiler Chicken Carcasses.

doi: 10.3390/pathogens13010030

Figure Lengend Snippet: Figure 6. Application of the HGMF-CH method using samples of broiler chicken carcass rinsates artificially contaminated with C. perfringens AHL 311 (tube 1, Table 2). (A) C. perfringens growth on a hydrophobic grid incubated on TSC agar plates after the filtration of a broiler chicken carcass rinsate sample artificially contaminated with C. perfringens AHL 311 (corresponding to sample tube 1). (B) Colonies showing positive hybridization signals on a nylon membrane printed from TSC agar plates with C. perfringens growth (A). Red squares are showing colonies demonstrating a positive hybridization signal.

Article Snippet: Three distinct DNA extractions were performed: two DNA extractions from the cpepositive C. perfringens AHL 311 control strain that yielded 27.9 ng/μL and 33.3 ng/μL of DNA and one DNA extraction from the cpe-negative ATCC 13124 C. perfringens reference strain yielding 28.0 ng/μL of DNA.

Techniques: Incubation, Filtration, Hybridization, Membrane

Journal: Pathogens (Basel, Switzerland)

Article Title: Addressing Current Challenges in Poultry Meat Safety: Development of a Cultivation and Colony Hybridization Approach to Recover Enterotoxigenic Clostridium perfringens from Broiler Chicken Carcasses.

doi: 10.3390/pathogens13010030

Figure Lengend Snippet: Figure 8. Application of the HGMF-CH method using samples of broiler chicken carcass rinsates artificially contaminated with C. perfringens AHL 311 (tube 2, Table 2). (A) C. perfringens growth on a hydrophobic grid incubated on TSC agar plates after the filtration of a broiler chicken carcass rinsate sample artificially contaminated with C. perfringens AHL 311. (B) Colonies showing positive hybridization signals on a nylon membrane printed from TSC agar plates with C. perfringens growth (A) were filtered. The positive control (cpe PCR product) appears on the nylon membrane. Red square is showing colonies demonstrating a positive hybridization signal.

Article Snippet: Three distinct DNA extractions were performed: two DNA extractions from the cpepositive C. perfringens AHL 311 control strain that yielded 27.9 ng/μL and 33.3 ng/μL of DNA and one DNA extraction from the cpe-negative ATCC 13124 C. perfringens reference strain yielding 28.0 ng/μL of DNA.

Techniques: Incubation, Filtration, Hybridization, Membrane, Positive Control

Journal: Pathogens (Basel, Switzerland)

Article Title: Addressing Current Challenges in Poultry Meat Safety: Development of a Cultivation and Colony Hybridization Approach to Recover Enterotoxigenic Clostridium perfringens from Broiler Chicken Carcasses.

doi: 10.3390/pathogens13010030

Figure Lengend Snippet: Figure 9. PCR analysis of the suspect C. perfringens colonies subcultured on sheep blood agar plates after recovery from TSC agar plates following the detection of corresponding positive hybridization signals on nylon membranes. The 233 bp fragments correspond to C. perfringens enterotoxin-encoding gene, cpe (tube 2, see Table 2).

Article Snippet: Three distinct DNA extractions were performed: two DNA extractions from the cpepositive C. perfringens AHL 311 control strain that yielded 27.9 ng/μL and 33.3 ng/μL of DNA and one DNA extraction from the cpe-negative ATCC 13124 C. perfringens reference strain yielding 28.0 ng/μL of DNA.

Techniques: Hybridization

Journal: Pathogens (Basel, Switzerland)

Article Title: Addressing Current Challenges in Poultry Meat Safety: Development of a Cultivation and Colony Hybridization Approach to Recover Enterotoxigenic Clostridium perfringens from Broiler Chicken Carcasses.

doi: 10.3390/pathogens13010030

Figure Lengend Snippet: Figure 10. Application of the DP-CH method using samples of broiler chicken carcass rinsates artificially contaminated with C. perfringens AHL 311 (tube 5, Table 2). (A) Bacterial growth on a TSC agar plate obtained from the analysis of tube 5. (B) Positive hybridization signals from colonies printed from the corresponding TSC agar plate (see in (A)) (tube 5, see Table 2). Red squares are showing colonies demonstrating a positive hybridization signal.

Article Snippet: Three distinct DNA extractions were performed: two DNA extractions from the cpepositive C. perfringens AHL 311 control strain that yielded 27.9 ng/μL and 33.3 ng/μL of DNA and one DNA extraction from the cpe-negative ATCC 13124 C. perfringens reference strain yielding 28.0 ng/μL of DNA.

Techniques: Hybridization

Journal: Pathogens (Basel, Switzerland)

Article Title: Addressing Current Challenges in Poultry Meat Safety: Development of a Cultivation and Colony Hybridization Approach to Recover Enterotoxigenic Clostridium perfringens from Broiler Chicken Carcasses.

doi: 10.3390/pathogens13010030

Figure Lengend Snippet: Figure 11. Application of the DP-CH method using samples of broiler chicken carcass rinsates artificially contaminated with C. perfringens AHL 311 (tube 6, Table 2). (A) Bacterial growth on a TSC agar plate obtained from the analysis of tube 6. (B) Positive hybridization signals from colonies printed from the corresponding TSC agar plate (see in (A)) and from the positive control (cpe PCR product) (tube 6, Table 2). Red square is showing colonies demonstrating a positive hybridization signal.

Article Snippet: Three distinct DNA extractions were performed: two DNA extractions from the cpepositive C. perfringens AHL 311 control strain that yielded 27.9 ng/μL and 33.3 ng/μL of DNA and one DNA extraction from the cpe-negative ATCC 13124 C. perfringens reference strain yielding 28.0 ng/μL of DNA.

Techniques: Hybridization, Positive Control

Journal: Pathogens (Basel, Switzerland)

Article Title: Addressing Current Challenges in Poultry Meat Safety: Development of a Cultivation and Colony Hybridization Approach to Recover Enterotoxigenic Clostridium perfringens from Broiler Chicken Carcasses.

doi: 10.3390/pathogens13010030

Figure Lengend Snippet: Figure 12. Application of the DP-CH method using samples of broiler chicken carcass rinsates artificially contaminated with C. perfringens AHL 311 (tube 7, Table 2). (A1–A4) Bacterial growth on a TSC agar plate obtained from the analysis of tube 7. (B1–B4) Positive hybridization signals from colonies printed from the corresponding TSC agar plate (see in (A1–A4)) and from the positive control (cpe PCR product). Red squares are showing colonies demonstrating a positive hybridization signal.

Article Snippet: Three distinct DNA extractions were performed: two DNA extractions from the cpepositive C. perfringens AHL 311 control strain that yielded 27.9 ng/μL and 33.3 ng/μL of DNA and one DNA extraction from the cpe-negative ATCC 13124 C. perfringens reference strain yielding 28.0 ng/μL of DNA.

Techniques: Hybridization, Positive Control

Journal: Pathogens (Basel, Switzerland)

Article Title: Addressing Current Challenges in Poultry Meat Safety: Development of a Cultivation and Colony Hybridization Approach to Recover Enterotoxigenic Clostridium perfringens from Broiler Chicken Carcasses.

doi: 10.3390/pathogens13010030

Figure Lengend Snippet: Figure 13. PCR analysis of the suspect C. perfringens colonies subcultured on sheep blood agar plates after recovery from TSC agar plates following the detection of corresponding positive hybridization signals on nylon membranes. Fragments of 233 bp correspond to C. perfringens enterotoxin-encoding gene, cpe (tube 7, see Table 2).

Article Snippet: Three distinct DNA extractions were performed: two DNA extractions from the cpepositive C. perfringens AHL 311 control strain that yielded 27.9 ng/μL and 33.3 ng/μL of DNA and one DNA extraction from the cpe-negative ATCC 13124 C. perfringens reference strain yielding 28.0 ng/μL of DNA.

Techniques: Hybridization